Abstract
Interleukin-4 (IL-4) is an immunoregulatory cytokine produced by activated T lymphocytes to promote the growth and differentiation of cells that participate in immune defense. This study demonstrates the rapid activation of a specific DNA binding factor by IL-4. The IL-4 nuclear-activated factor (IL-4 NAF) appeared within minutes of IL-4 stimulation and recognized a specific DNA sequence found in the promoters of IL-4-responsive genes. Activation of this putative transcription factor required tyrosine phosphorylation, and antibodies specific for phosphotyrosine recognize the IL-4 NAF-DNA complex. Thus, IL-4 appears to transduce a signal to the nucleus through tyrosine phosphorylation of a latent DNA binding factor.
Keywords
Tyrosine phosphorylationPhosphorylationTranscription factorBiologyMolecular biologyDNA binding siteCell biologyPromoterGeneBiochemistryGene expression
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Publication Info
- Year
- 1993
- Type
- article
- Volume
- 262
- Issue
- 5137
- Pages
- 1265-1267
- Citations
- 254
- Access
- Closed
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Cite This
Helen Kotanides,
Nancy C. Reich
(1993).
Requirement of Tyrosine Phosphorylation for Rapid Activation of a DNA Binding Factor by IL-4.
Science
, 262
(5137)
, 1265-1267.
https://doi.org/10.1126/science.7694370
Identifiers
- DOI
- 10.1126/science.7694370