Abstract
Certain phenomena affecting contrast obtained from tissue sections with the electron microscope have been investigated and a technique is described for reducing destruction by the electron beam of fine details in sections. It has been concluded that loss of embedding material is slightly higher at exposed surfaces of sections than it is at surfaces covered by substrate film. Covering of both surfaces of sections with thin films of formvar, collodion, or carbon materially improves the general appearance, reduces distortion, and sometimes reduces loss of tissue mass from the section as result of exposure to the electron beam. This improvement is considered to result from the relatively high melting-point of the covering films which serve to eliminate or reduce surface-tension or other forces operating in methacrylate softened by the electron beam.
Keywords
Electron microscopeMaterials scienceCathode raySubstrate (aquarium)Electron beam-induced depositionOpticsThin filmBeam (structure)ElectronScanning electron microscopeComposite materialNanotechnologyScanning transmission electron microscopyBiologyPhysics
Related Publications
XPS and STM Investigation of the Passive Film Formed on Cr(110) Single‐Crystal Surfaces
A combined ex situ x‐ray photoelectron spectroscopy (XPS) and scanning tunneling microscopy (STM) investigation of the passive films formed on Cr(110) single‐crystal surfaces in...
Epoxy Resins in Electron Microscopy
A method of embedding biological specimens in araldite 502 (Ciba) has been developed for materials available in the United States. Araldite-embedded tissues are suitable for ele...
IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS
Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenient embedding methods for electron microscopy. The sections are ...
Atomic force microscopy and surface-enhanced Raman spectroscopy. I. Ag island films and Ag film over polymer nanosphere surfaces supported on glass
The surface roughness and nanometer scale structure of Ag films used for surface-enhanced Raman scattering (SERS) are characterized using atomic force microscopy (AFM). Two impo...
Patterning of highly oriented pyrolytic graphite by oxygen plasma etching
Patterning of highly oriented pyrolytic graphite (HOPG) was demonstrated by oxygen plasma etching of lithographically patterned substrates. Periodic arrays of islands, or holes ...
Publication Info
- Year
- 1957
- Type
- article
- Volume
- 3
- Issue
- 6
- Pages
- 1017-1022
- Citations
- 91
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
91
OpenAlex
Cite This
Michael L. Watson
(1957).
REDUCTION OF HEATING ARTIFACTS IN THIN SECTIONS EXAMINED IN THE ELECTRON MICROSCOPE.
The Journal of Cell Biology
, 3
(6)
, 1017-1022.
https://doi.org/10.1083/jcb.3.6.1017
Identifiers
- DOI
- 10.1083/jcb.3.6.1017