Abstract
Quantification of the level of expression for a specific gene in samples obtained during various experimental conditions is becoming increasingly important as attempts are made to gain knowledge relating to how these altered conditions affect cells at the molecular level. Traditionally, an estimate of the copy number of a gene transcription product in a sample was made by employing traditional Northern blot, dot-blot hybridization, or solution hybridization techniques. These techniques are labor-intensive and usually require microgram quantities of total RNA in order to detect specific mRNAs. The low sensitivity of these assays makes it difficult to estimate differences in expression less than fourfold. In addition, genes with low levels of expression often cannot be detected at all by these assays.
Keywords
Dot blotMolecular biologyPolymerase chain reactionNorthern blotBiologyGene expressionGeneSouthern blotReverse transcription polymerase chain reactionTranscription (linguistics)Messenger RNAReal-time polymerase chain reactionGenetics
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Publication Info
- Year
- 2003
- Type
- review
- Volume
- 110
- Pages
- 43-62
- Citations
- 95
- Access
- Closed
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Cite This
William H. Karge,
Erirst J. Schaefer,
José M. Ordovás
(2003).
Quantification of inRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method.
Humana Press eBooks
, 110
, 43-62.
https://doi.org/10.1385/1-59259-582-0:43
Identifiers
- DOI
- 10.1385/1-59259-582-0:43