Abstract
The reverse transcription polymerase chain reaction (RT-PCR) was used to assess the induction of mRNA of the proinflammatory cytokines IL-1 beta, IL-6 and TNF alpha in the spleen, pituitary, hypothalamus and hippocampus of mice after an intraperitoneal injection of lipopolysaccharide (LPS, 10 micrograms/mouse). The kinetics of cytokine gene expression induced by peripheral LPS in the pituitary and brain structures were different from that observed in the spleen. For IL-1 beta the dose-response curve was also measured and also found to be different. These results support the idea that one pathway by which peripheral immune stimuli affect brain functions includes local synthesis of proinflammatory cytokines in certain brain structures.
Keywords
Proinflammatory cytokineLipopolysaccharideBiologyEndocrinologyCytokineInternal medicineSpleenImmune systemHypothalamusPituitary glandTumor necrosis factor alphaPeripheralInflammationImmunologyMedicineHormone
MeSH Terms
AnimalsBase SequenceBrainDose-Response RelationshipDrugGene Expression RegulationHippocampusHypothalamusInflammationInterleukin-1Interleukin-6KineticsLipopolysaccharidesMaleMiceMolecular Sequence DataNerve Tissue ProteinsPituitary GlandSpleenTumor Necrosis Factor-alphabeta 2-Microglobulin
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Publication Info
- Year
- 1994
- Type
- article
- Volume
- 27
- Issue
- 1
- Pages
- 157-162
- Citations
- 496
- Access
- Closed
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Cite This
Sophie Layé,
Patricia Parnet,
Emmanuelle Goujon
et al.
(1994).
Peripheral administration of lipopolysaccharide induces the expression of cytokine transcripts in the brain and pituitary of mice.
Molecular Brain Research
, 27
(1)
, 157-162.
https://doi.org/10.1016/0169-328x(94)90197-x
Identifiers
- DOI
- 10.1016/0169-328x(94)90197-x
- PMID
- 7877446