Abstract

Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for investigations of genomes, transcriptomes, epigenomes and epitranscriptomes. Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the development of new nanopores, base-calling methods and experimental protocols tailored to particular applications.

Keywords

Nanopore sequencingNanoporeComputational biologyDNA sequencingGenomeComputer scienceSequence assemblyExploitGenomicsBiologyNanotechnologyDNAGeneTranscriptomeGeneticsMaterials scienceComputer securityGene expression

MeSH Terms

Computational BiologyHigh-Throughput Nucleotide SequencingNanopore SequencingNanoporesSequence AnalysisDNATechnology

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Publication Info

Year
2021
Type
review
Volume
39
Issue
11
Pages
1348-1365
Citations
1536
Access
Closed

External Links

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Cite This

Yunhao Wang, Yue Zhao, Audrey Bollas et al. (2021). Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology , 39 (11) , 1348-1365. https://doi.org/10.1038/s41587-021-01108-x

Identifiers

DOI
10.1038/s41587-021-01108-x
PMID
34750572
PMCID
PMC8988251

Data Quality

Data completeness: 86%