miR-200b mediates post-transcriptional repression of ZFHX1B

Abstract

MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFβ signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3′-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.

Keywords

Psychological repressionBiologyRepressormicroRNAThree prime untranslated regionEndogenyCell biologyUntranslated regionTranscriptional regulationRegulation of gene expressionMessenger RNATransition (genetics)GeneticsGene expressionGeneEndocrinology

Affiliated Institutions

University of Copenhagen DK

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Publication Info

Year: 2007
Type: article
Volume: 13
Issue: 8
Pages: 1172-1178
Citations: 167
Access: Closed

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APA Style

                            
                                
                                    Nanna R. Christoffersen, 
                                
                                    Asli Silahtaroglu, 
                                
                                    Ulf Andersson Ørom
                                
                                et al.
                            
                            (2007). 
                            miR-200b mediates post-transcriptional repression of ZFHX1B. 
                            RNA
                            , 13
                            (8)
                            , 1172-1178.
                            https://doi.org/10.1261/rna.586807
                        

Identifiers

DOI: 10.1261/rna.586807