Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours

Abstract

Mutations in the p53 tumour-suppressor gene are the most frequently observed genetic lesions in human cancers. To investigate the role of the p53 gene in mammalian development and tumorigenesis, a null mutation was introduced into the gene by homologous recombination in murine embryonic stem cells. Mice homozygous for the null allele appear normal but are prone to the spontaneous development of a variety of neoplasms by 6 months of age. These observations indicate that a normal p53 gene is dispensable for embryonic development, that its absence predisposes the animal to neoplastic disease, and that an oncogenic mutant form of p53 is not obligatory for the genesis of many types of tumours.

Keywords

BiologyCarcinogenesisNull alleleAlleleSuppressorGeneGene targetingMutantTumor suppressor geneMutationEmbryonic stem cellHomologous recombinationCancer researchGeneticsHomologous chromosome

MeSH Terms

AllelesAnimalsBase SequenceBlastocystBlottingSouthernDNAExonsFemaleGenesp53Genetic VectorsHeterozygoteHomozygoteMaleMiceMiceInbred C57BLMiceInbred CBAMiceTransgenicMolecular Sequence DataMutationNeoplasmsExperimentalPolymerase Chain ReactionRNAMessengerStem CellsTumor Suppressor Protein p53

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Publication Info

Year: 1992
Type: article
Volume: 356
Issue: 6366
Pages: 215-221
Citations: 4656
Access: Closed

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APA Style

                            
                                
                                    Lawrence A. Donehower, 
                                
                                    Michele Harvey, 
                                
                                    Betty L. Slagle
                                
                                et al.
                            
                            (1992). 
                            Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours. 
                            Nature
                            , 356
                            (6366)
                            , 215-221.
                            https://doi.org/10.1038/356215a0
                        

Identifiers

DOI: 10.1038/356215a0
PMID: 1552940

Data Quality

Data completeness: 81%