Abstract
New mass spectrometry techniques, such as electrospray ionization (ESI), allow the study of large biomolecules and peptide mixtures. The data produced are complex and interpretation can be a long and tedious process. A new suite of data-processing software is described which allows many of these operations to be carried out in a rapid, automated way. Software is described for the deconvolution of the spectra of multiply charged ions, for both pure compounds and mixtures. The rapid peptide mapping of protein digests from h.p.l.c.-m.s. data and peptide sequence confirmation from multiple-stage (MS)-m.s. data using tandem quadrupole m.s. are also described. In addition preliminary results are presented on first principle sequencing of unknown peptides from MS-m.s. experiments.
Keywords
Fast atom bombardmentElectrospray mass spectrometryMass spectrometryChemistryElectrosprayChromatography
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Publication Info
- Year
- 1991
- Type
- article
- Volume
- 19
- Issue
- 4
- Pages
- 957-962
- Citations
- 9
- Access
- Closed
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Cite This
Peter Watkins,
Ian Jardine,
Joe X. G. Zhou
(1991).
Mass spectrometry software for biochemical analysis in electrospray and fast atom bombardment modes.
Biochemical Society Transactions
, 19
(4)
, 957-962.
https://doi.org/10.1042/bst0190957
Identifiers
- DOI
- 10.1042/bst0190957