Abstract

A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis (NANBH) agent and screened with serum from a patient diagnosed with NANBH. A complementary DNA clone was isolated that was shown to encode an antigen associated specifically with NANBH infections. This clone is not derived from host DNA but from an RNA molecule present in NANBH infections that consists of at least 10,000 nucleotides and that is positive-stranded with respect to the encoded NANBH antigen. These data indicate that this clone is derived from the genome of the NANBH agent and are consistent with the agent being similar to the togaviridae or flaviviridae. This molecular approach should be of great value in the isolation and characterization of other unidentified infectious agents.

Keywords

clone (Java method)Complementary DNABiologyVirologyDNAGenomeMolecular biologyGeneticsGene

MeSH Terms

AnimalsAntigensViralBacteriophage lambdaDNAHepatitis AntibodiesHepatitis CHepatitis VirusesHepatitisViralHumanImmunoblottingNucleic Acid HybridizationPan troglodytesProtein BiosynthesisRNAViral

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Publication Info

Year
1989
Type
article
Volume
244
Issue
4902
Pages
359-362
Citations
6866
Access
Closed

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Cite This

Qui‐Lim Choo, George Kuo, Amy J. Weiner et al. (1989). Isolation of a cDNA cLone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome. Science , 244 (4902) , 359-362. https://doi.org/10.1126/science.2523562

Identifiers

DOI
10.1126/science.2523562
PMID
2523562

Data Quality

Data completeness: 86%