Abstract
The erythrocytic stages of Plasmodium falciparum from Aotus trivirgatus were grown in Mill Hill medium. Significant invasion of erythrocytes was obtained by a subculture technique. This invasion, by parasites grown in Aotus erythrocytes, was greater when human erythrocytes were added to the cultures than when parasites were allowed to invade Aotus erythrocytes. This difference appeared to be a function of the properties of the erythrocyte and was not serum dependent.
Keywords
Affiliated Institutions
Related Publications
Human Malaria Parasites in Continuous Culture
Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent...
Culture of<i>Plasmodium falciparum in vitro</i>: a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area
A technique is described for obtaining consistent reinvasion and multiplication of the erythrocytic stages of Plasmodium falciparum from humans in vitro . Subsequent to this, P....
A New Method for Intraerythrocytic Cultivation of Malaria Parasites (<i>Plasmodium coatneyi</i> and <i>P. falciparum</i>)
SYNOPSIS. Infected blood (from the rhesus monkey with P. coatneyi or an Aotus trivirgatus monkey with P. falciparum ) was placed in a special vial equipped with an overflow tube...
Effects of Red Cell Extract on In vitro Growth and Multiplication of Malarial Parasites
An extract of red blood cells was prepared from monkey blood. Red cell extract (RCE) of Aotus monkeys was beneficial for the in-vitro cultivation of 2 strains of P. falciparum. ...
THE CULTIVATION OF MALARIAL PLASMODIA (PLASMODIUM VIVAX AND PLASMODIUM FALCIPARUM) IN VITRO
The asexual cycle of Plasmodium vivax and Plasmodium falciparum has been cultivated in vitro in human blood. The parasites have been grown also in red blood cells in the presenc...
Publication Info
- Year
- 1975
- Type
- article
- Volume
- 71
- Issue
- 3
- Pages
- 433-436
- Citations
- 11
- Access
- Closed
External Links
Social Impact
Social media, news, blog, policy document mentions
Citation Metrics
Cite This
Identifiers
- DOI
- 10.1017/s0031182000047193