Abstract
Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus .
Keywords
Plasmodium falciparumMalariaBiologyParasite hostingMalarial parasitesCarbon dioxideMicrobiologyEcologyImmunology
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Publication Info
- Year
- 1976
- Type
- article
- Volume
- 193
- Issue
- 4254
- Pages
- 673-675
- Citations
- 7834
- Access
- Closed
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Cite This
William Trager,
James B. Jensen
(1976).
Human Malaria Parasites in Continuous Culture.
Science
, 193
(4254)
, 673-675.
https://doi.org/10.1126/science.781840
Identifiers
- DOI
- 10.1126/science.781840