Abstract
Human alpha- and beta-interferons (IFNs) stimulate rapid but transient increases in transcription from a set of previously quiescent genes. Protein synthesis is not required for initial stimulation, but duration of the response is limited to a few hours by a process requiring synthesis of new proteins. An IFN-stimulated response element (ISRE) was identified 5' to an inducible gene by deletion analysis and point mutagenesis, and sequence comparisons with other promoters defined the consensus element YAGTTTC(A/T)YTTTYCC. Two classes of IFN-inducible nuclear factors were found that bind to the ISRE. The most rapidly induced factor appeared without new protein synthesis, whereas a second factor required active protein synthesis for its appearance and maintenance. The kinetics of appearance and loss of these binding activities correlate with the activation and repression of IFN-stimulated genes. These different IFN-activated or induced factors may bind sequentially to the same essential promoter element to first increase and then repress transcription.
Keywords
BiologyResponse elementTranscription factorPromoterPsychological repressionDNA-binding proteinGeneTranscription (linguistics)MutantMolecular biologyInterferon regulatory factorsIRF1MutagenesisInterferonCell biologyGeneticsGene expression
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Publication Info
- Year
- 1988
- Type
- article
- Volume
- 2
- Issue
- 4
- Pages
- 383-393
- Citations
- 572
- Access
- Closed
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Cite This
David E. Levy,
Daniel S. Kessler,
Richard Pine
et al.
(1988).
Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control..
Genes & Development
, 2
(4)
, 383-393.
https://doi.org/10.1101/gad.2.4.383
Identifiers
- DOI
- 10.1101/gad.2.4.383