Abstract

Overlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detectable in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.

Keywords

NucleotideDNABiologyGeneMolecular biologyLocus (genetics)GeneticsCloning (programming)genomic DNA

Affiliated Institutions

Related Publications

Publication Info

Year
1989
Type
article
Volume
245
Issue
4922
Pages
1066-1073
Citations
7361
Access
Closed

External Links

View on DOI.org

Social Impact

Altmetric
PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

7361
OpenAlex

Cite This

John R. Riordan, Johanna M. Rommens, Bat-Sheva Kerem et al. (1989). Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA. Science , 245 (4922) , 1066-1073. https://doi.org/10.1126/science.2475911

Identifiers

DOI
10.1126/science.2475911