Abstract

Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4 , interleukin-13 , and interleukin-5 , spread over 120 kilobases.

Keywords

BiologyRegulatory sequenceConserved sequenceGeneticsRegulatorGeneSequence (biology)Computational biologyIdentification (biology)Sequence analysisRegulation of gene expressionPeptide sequence

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Publication Info

Year
2000
Type
article
Volume
288
Issue
5463
Pages
136-140
Citations
799
Access
Closed

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Gabriela G. Loots, Richard M. Locksley, Catherine M. Blankespoor et al. (2000). Identification of a Coordinate Regulator of Interleukins 4, 13, and 5 by Cross-Species Sequence Comparisons. Science , 288 (5463) , 136-140. https://doi.org/10.1126/science.288.5463.136

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DOI
10.1126/science.288.5463.136