Genome‐Scale Mapping of DNase I Hypersensitivity

Abstract

Abstract DNase I‐seq is a global and high‐resolution method that uses the nonspecific endonuclease DNase I to map chromatin accessibility. These accessible regions, designated as DNase I hypersensitive sites (DHSs), define the regulatory features, (e.g., promoters, enhancers, insulators, and locus control regions) of complex genomes. In this unit, methods are described for nuclei isolation, digestion of nuclei with limiting concentrations of DNase I, and the biochemical fractionation of DNase I hypersensitive sites in preparation for high‐throughput sequencing. DNase I‐seq is an unbiased and robust method that is not predicated on an a priori understanding of regulatory patterns or chromatin features. Curr. Protoc. Mol. Biol . 103:21.27.1–21.27.20. © 2013 by John Wiley & Sons, Inc.

Keywords

Hypersensitive siteChromatinDNase I hypersensitive siteDeoxyribonuclease IBiologyGenomeEnhancerNucleaseLocus control regionComputational biologyGeneticsDNAMolecular biologyGeneBase sequenceTranscription factor

Affiliated Institutions

University of Washington US

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Publication Info

Year: 2013
Type: article
Volume: 103
Issue: 1
Pages: Unit 21.27-Unit 21.27
Citations: 93
Access: Closed

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APA Style

                            
                                
                                    Sam John, 
                                
                                    Peter J. Sabo, 
                                
                                    Theresa K. Canfield
                                
                                et al.
                            
                            (2013). 
                            Genome‐Scale Mapping of DNase I Hypersensitivity. 
                            Current Protocols in Molecular Biology
                            , 103
                            (1)
                            , Unit 21.27-Unit 21.27.
                            https://doi.org/10.1002/0471142727.mb2127s103
                        

Identifiers

DOI: 10.1002/0471142727.mb2127s103