Genetic Definition and Sequence Analysis of Arabidopsis Centromeres

Abstract

High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These regions exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180–base pair repeats. Unexpectedly, the DNA within the centromeres was not merely structural but also encoded several expressed genes. The regions flanking the centromeres were densely populated by repetitive elements yet experienced normal levels of recombination. The genetically defined centromeres were well conserved among Arabidopsis ecotypes but displayed limited sequence homology between different chromosomes, excluding repetitive DNA. This investigation provides a platform for dissecting the role of individual sequences in centromeres in higher eukaryotes.

Keywords

CentromereArabidopsisBiologyGeneticsRepeated sequenceHomology (biology)ChromosomeGeneSatellite DNADNAEvolutionary biologyComputational biologyGenomeMutant

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Publication Info

Year: 1999
Type: article
Volume: 286
Issue: 5449
Pages: 2468-2474
Citations: 414
Access: Closed

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APA Style

                            
                                
                                    Gregory P. Copenhaver, 
                                
                                    Kathryn Nickel, 
                                
                                    Takashi Kuromori
                                
                                et al.
                            
                            (1999). 
                            Genetic Definition and Sequence Analysis of <i>Arabidopsis</i> Centromeres. 
                            Science
                            , 286
                            (5449)
                            , 2468-2474.
                            https://doi.org/10.1126/science.286.5449.2468
                        

Identifiers

DOI: 10.1126/science.286.5449.2468

Genetic Definition and Sequence Analysis of <i>Arabidopsis</i> Centromeres