An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana†

Abstract

As part of the goal to generate a detailed transcript map for Arabidopsis thaliana , 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random in libraries prepared from different sources of plant material: developing siliques, etiolated seedlings, flower buds, and cultured cells. Eight hundred and ninety‐five different genes could be identified, 32% of which showed significant similarity to existing sequences in Arabidopsis and an array of other organisms. These sequences in combination with their positioning on the Arabidopsis genetic map will not only constitute a new set of molecular markers for genome analysis in Arabidopsis but also provide a direct route for the in vivo analysis of their gene products. The sequences have been made available to the public databases.

Keywords

Arabidopsis thalianaSequence (biology)BiologyComputational biologyArabidopsisGeneticsGene

Affiliated Institutions

Related Publications

Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones

Trent Newman , Frans J. de Bruijn , P. Green +9 more

High-throughput automated partial sequencing of anonymous cDNA clones provides a method to survey the repertoire of expressed genes from an organism. Comparison of the coding ca...

1994 PLANT PHYSIOLOGY 635 citations

Arabidopsis thaliana and Plant Molecular Genetics

Elliot M. Meyerowitz , Robert E. Pruitt

Arabidopsis thaliana is a small flowering plant with various properties that make it an excellent organism for experiments in molecular genetics. These properties include having...

1985 Science 252 citations

Genome-wide identification of chromosomal regions of increased tumor expression by transcriptome analysis.

Yan Zhou , Shiuh-Ming Luoh , Yan Zhang +5 more

Genes up-regulated in tumor cells provide attractive anticancer therapeutic targets. Although the general underlying mechanism for the increased expression in tumors is unknown,...

2003 PubMed 51 citations

Identification and map position of YAC clones comprising one‐third of the Arabidopsis genome.

Inhwan Hwang , Takayuki Kohchi , Hauge Bm +7 more

Summary YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Bas...

1991 The Plant Journal 66 citations

Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA

Monique Gardes , Thomas J. White , J. André Fortin +2 more

We used the polymerase chain reaction (PCR) to amplify specific regions of the nuclear and mitochondrial genomes of fungi using DNA extracted from pure cultures as well as that ...

1991 Canadian Journal of Botany 473 citations

Publication Info

Year: 1993
Type: article
Volume: 4
Issue: 6
Pages: 1051-1061
Citations: 235
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana†

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

235

OpenAlex

Cite This

APA Style

                            
                                
                                    Hermanus Höfte, 
                                
                                    Thierry T. Desprez, 
                                
                                    J. Anselem
                                
                                et al.
                            
                            (1993). 
                            An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from <i>Arabidopsis thaliana</i><sup>†</sup>. 
                            The Plant Journal
                            , 4
                            (6)
                            , 1051-1061.
                            https://doi.org/10.1046/j.1365-313x.1993.04061051.x
                        

Identifiers

DOI: 10.1046/j.1365-313x.1993.04061051.x