Abstract
Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.
Keywords
SpheroplastBiologyAuxotrophyMating typeSaccharomyces cerevisiaeZygotePloidyReversionPolyethylene glycolYeastMating of yeastCell fusionMatingBiochemistrySaccharomycesMicrobiologyGeneticsCellGeneEscherichia coliPhenotype
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Publication Info
- Year
- 1977
- Type
- article
- Volume
- 130
- Issue
- 2
- Pages
- 946-947
- Citations
- 145
- Access
- Closed
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Cite This
P. van Solingen,
J.B. van der Plaat
(1977).
Fusion of yeast spheroplasts.
Journal of Bacteriology
, 130
(2)
, 946-947.
https://doi.org/10.1128/jb.130.2.946-947.1977
Identifiers
- DOI
- 10.1128/jb.130.2.946-947.1977