Abstract
The GAL1 and GAL10 genes of Saccharomyces cerevisiae are divergently transcribed, with 606 base pairs of DNA separating their transcription initiation sites. These two genes are stringently coregulated: their expression is induced ca. 1,000-fold in cells growing on galactose and is repressed by growth on glucose. The nucleotide sequence of the region of DNA between these genes and the precise sites of transcription initiation are presented here. The most notable feature of the nucleotide sequence of this region is a 108-base-pair guanine-plus-cytosine-rich stretch of DNA located approximately in the middle of the region between GAL1 and GAL10. Analysis of the effects of mutations that alter the region between these two genes, constructed in vitro or selected in vivo, suggest that these guanine-plus-cytosine-rich sequences are required for the expression of both genes. The region of DNA between GAL1 and GAL10 is sufficient for regulation of expression of these genes: fusion of the region to the yeast HIS3 gene places HIS3 under GAL control.
Keywords
BiologySaccharomyces cerevisiaeGeneticsPromoterComputational biologyGeneGene expression
Affiliated Institutions
Related Publications
Search for the optimal sequence of the ribosome binding site by random oligonucleotide-directed mutagenesis
Synthetic DNA duplexes corresponding to the ribosome binding site (RBS) were synthesized through the phosphite method on solid support. The synthetic RBS DNA with partial random...
Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein
We describe a new method for accurately defining the sequence recognition properties of DNA-binding proteins by selecting high-affinity binding sites from random-sequence DNA. T...
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7 promoter. Partially sin...
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in t...
Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in t...
Publication Info
- Year
- 1984
- Type
- article
- Volume
- 4
- Issue
- 8
- Pages
- 1440-1448
- Citations
- 949
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
949
OpenAlex
Cite This
Mark Johnston,
Ronald W. Davis
(1984).
Sequences That Regulate the Divergent <i>GAL1-GAL10</i> Promoter in <i>Saccharomyces cerevisiae</i>.
Molecular and Cellular Biology
, 4
(8)
, 1440-1448.
https://doi.org/10.1128/mcb.4.8.1440-1448.1984
Identifiers
- DOI
- 10.1128/mcb.4.8.1440-1448.1984