Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

Abstract

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

Keywords

TMPRSS2Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Isolation (microbiology)Coronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakVirologySars virusBetacoronavirusBiologyComputational biologyMedicineMicrobiologyOutbreakInternal medicineInfectious disease (medical specialty)Disease

MeSH Terms

AnimalsBetacoronavirusCOVID-19Cell LineChlorocebus aethiopsCoronavirus InfectionsDisease OutbreaksHumansPandemicsPneumoniaViralRNAViralSARS-CoV-2Serine EndopeptidasesVero CellsVirus Cultivation

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Publication Info

Year: 2020
Type: article
Volume: 117
Issue: 13
Pages: 7001-7003
Citations: 1594
Access: Closed

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APA Style

                            
                                
                                    Shutoku Matsuyama, 
                                
                                    Naganori Nao, 
                                
                                    Kazuya Shirato
                                
                                et al.
                            
                            (2020). 
                            Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. 
                            Proceedings of the National Academy of Sciences
                            , 117
                            (13)
                            , 7001-7003.
                            https://doi.org/10.1073/pnas.2002589117
                        

Identifiers

DOI: 10.1073/pnas.2002589117
PMID: 32165541
PMCID: PMC7132130

Data Quality

Data completeness: 90%