Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.

Jean‐Paul Behr; Barbara Demeneix; Jean-Philippe Loeffler; José Pérez-Mutul

doi:10.1073/pnas.86.18.6982

Abstract

A general and efficient transfection procedure, based on compacted lipopolyamine-coated plasmids, has been developed. The active species is obtained by simple addition of excess synthetic lipospermine solution to the DNA and binds within minutes to the cell membrane. This technique has been developed on endocrine cells of the intermediate lobe of the pituitary as a general tool for physiological work on primary cells; it is not toxic and does not interfere with physiological regulations in melanotrope cells. A variety of eukaryotic cell cultures also have been transfected with success for transient and stable expression.

Keywords

TransfectionPrimary cellDNAEnteroendocrine cellPlasmidCell biologyExogenous DNABiologyGeneCell cultureCellMolecular biologyEndocrine systemChemistryBiochemistryGeneticsHormone

Affiliated Institutions

Laboratoire de Chimie Organique FR

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Publication Info

Year: 1989
Type: article
Volume: 86
Issue: 18
Pages: 6982-6986
Citations: 717
Access: Closed

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APA Style

                            
                                
                                    Jean‐Paul Behr, 
                                
                                    Barbara Demeneix, 
                                
                                    Jean-Philippe Loeffler
                                
                                et al.
                            
                            (1989). 
                            Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.. 
                            Proceedings of the National Academy of Sciences
                            , 86
                            (18)
                            , 6982-6986.
                            https://doi.org/10.1073/pnas.86.18.6982
                        

Identifiers

DOI: 10.1073/pnas.86.18.6982