Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components

Abstract

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Keywords

Lipoteichoic acidPeptidoglycanTLR2TLR4BiologyLipopolysaccharideMicrobiologyBacterial cell structureToll-like receptorIn vivoCell envelopeGram-negative bacteriaCell wallStaphylococcus aureusReceptorBacteriaImmunologyInnate immune systemInflammationEscherichia coliBiochemistryGene

MeSH Terms

AnimalsAntigensBacterialCell WallCorynebacterium diphtheriaeDrosophila ProteinsEscherichia coliFemaleGram-Negative BacteriaGram-Positive BacteriaInterleukin-1 Receptor-Associated KinasesLipid ALipopolysaccharidesMacrophagesPeritonealMaleMembrane GlycoproteinsMiceMiceInbred C57BLMiceKnockoutNF-kappa BNocardiaPeptidoglycanProtein IsoformsProtein KinasesReceptorsCell SurfaceSalmonellaSignal TransductionSpecies SpecificityStaphylococcus aureusTeichoic AcidsToll-Like Receptor 2Toll-Like Receptor 4Toll-Like Receptors

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Publication Info

Year: 1999
Type: article
Volume: 11
Issue: 4
Pages: 443-451
Citations: 3334
Access: Closed

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APA Style

                            
                                
                                    Osamu Takeuchi, 
                                
                                    Katsuaki Hoshino, 
                                
                                    Taro Kawai
                                
                                et al.
                            
                            (1999). 
                            Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components. 
                            Immunity
                            , 11
                            (4)
                            , 443-451.
                            https://doi.org/10.1016/s1074-7613(00)80119-3
                        

Identifiers

DOI: 10.1016/s1074-7613(00)80119-3
PMID: 10549626

Data Quality

Data completeness: 86%