Dependence of Fluorescence Intensity on the Spectral Overlap between Fluorophores and Plasmon Resonant Single Silver Nanoparticles

Yeechi Chen; Keiko Munechika; David S. Ginger

doi:10.1021/nl062795z

Abstract

We investigate the fluorescence from dyes coupled to individual DNA-functionalized metal nanoparticles. We use single-particle darkfield scattering and fluorescence microscopy to correlate the fluorescence intensity of the dyes with the localized surface plasmon resonance (LSPR) spectra of the individual metal nanoparticles to which they are attached. For each of three different dyes, we observe a strong correlation between the fluorescence intensity of the dye and the degree of spectral overlap with the plasmon resonance of the nanoparticle. On average, we observe the brightest fluorescence from dyes attached to metal nanoparticles that have a LSPR scattering peak approximately 40-120 meV higher in energy than the emission peak of the fluorophore. These results should prove useful for understanding and optimizing metal-enhanced fluorescence.

Keywords

FluorophoreFluorescenceSurface plasmon resonanceNanoparticlePlasmonFluorescence in the life sciencesMaterials scienceResonance fluorescenceFluorescence correlation spectroscopySilver nanoparticleLocalized surface plasmonFluorescence cross-correlation spectroscopyScatteringAnalytical Chemistry (journal)PhotochemistryChemistryFluorescence spectroscopyNanotechnologyOpticsOptoelectronics

Affiliated Institutions

University of Washington US

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Publication Info

Year: 2007
Type: article
Volume: 7
Issue: 3
Pages: 690-696
Citations: 676
Access: Closed

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APA Style

                            
                                    Yeechi Chen, 
                                
                                    Keiko Munechika, 
                                
                                    David S. Ginger
                                
                            (2007). 
                            Dependence of Fluorescence Intensity on the Spectral Overlap between Fluorophores and Plasmon Resonant Single Silver Nanoparticles. 
                            Nano Letters
                            , 7
                            (3)
                            , 690-696.
                            https://doi.org/10.1021/nl062795z

Identifiers

DOI: 10.1021/nl062795z