Abstract

Angiogenesis depends on cytokines and vascular cell adhesion events. Two cytokine-dependent pathways of angiogenesis were shown to exist and were defined by their dependency on distinct vascular cell integrins. In vivo angiogenesis in corneal or chorioallantoic membrane models induced by basic fibroblast growth factor or by tumor necrosis factor-α depended on α v β 3 , whereas angiogenesis initiated by vascular endothelial growth factor, transforming growth factor-α, or phorbol ester depended on α v β 5 . Antibody to each integrin selectively blocked one of these pathways, and a cyclic peptide antagonist of both integrins blocked angiogenesis stimulated by each cytokine tested. These pathways are further distinguished by their sensitivity to calphostin C, an inhibitor of protein kinase C that blocked angiogenesis potentiated by α v β 5 but not by α v β 3 .

Keywords

AngiogenesisIntegrinCell biologyBasic fibroblast growth factorCytokineEndothelial stem cellVascular endothelial growth factorVascular endothelial growth factor ABiologySignal transductionAlpha-v beta-3Growth factorChemistryCancer researchImmunologyReceptorVitronectinBiochemistryIn vitroVEGF receptors

MeSH Terms

AnimalsAntibodiesMonoclonalChick EmbryoCorneaEndothelial Growth FactorsEnzyme InhibitorsFibroblast Growth Factor 2IntegrinsLymphokinesNaphthalenesNeovascularizationPathologicNeovascularizationPhysiologicPeptidesCyclicProtein Kinase CRabbitsReceptorsVitronectinTetradecanoylphorbol AcetateTransforming Growth Factor alphaTumor Necrosis Factor-alphaVascular Endothelial Growth Factor AVascular Endothelial Growth Factors

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Publication Info

Year
1995
Type
article
Volume
270
Issue
5241
Pages
1500-1502
Citations
1311
Access
Closed

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Cite This

Martin Friedlander, Peter C. Brooks, Robert W. Shaffer et al. (1995). Definition of Two Angiogenic Pathways by Distinct α <sub>v</sub> Integrins. Science , 270 (5241) , 1500-1502. https://doi.org/10.1126/science.270.5241.1500

Identifiers

DOI
10.1126/science.270.5241.1500
PMID
7491498

Data Quality

Data completeness: 81%