Abstract
Abstract An immune response can deviate toward either a Th1- or Th2-like response. In this work we examine the contribution that activated macrophages and IgG Abs make toward this deviation. The use of activated macrophages as APCs resulted in a strong polarized T cell response that was predominated by IFN-γ. However, when Ag was targeted to FcγRs on these macrophages, the T cell response was reversed and biased toward a Th2-like response. This Th2-like phenotype was stable and was retained when the T cells were subsequently restimulated under nonbiasing conditions. The T cell biasing and its reversal via FcγR was also observed in vivo. Mice vaccinated with IgG-opsonized OVA made high levels of IgG Ab of the IgG1 isotype. These studies demonstrate that the ligation of FcγR on activated macrophages can reverse the Th1 biasing that occurs as a result of innate immune responses to microbial products.
Keywords
OpsoninImmune systemImmunologyAntigenMacrophageIsotypeBiasingCell biologyReceptorFc receptorBiologyAntibodyIn vitroBiochemistryMonoclonal antibody
MeSH Terms
AdjuvantsImmunologicAnimalsAntibody SpecificityAntigen-Antibody ComplexAntigen-Presenting CellsAntigensCytokinesImmunityActiveImmunityCellularImmunoglobulin GInjectionsIntraperitonealLipid AMacrophage ActivationMacrophagesMiceMiceInbred BALB CMiceKnockoutMiceTransgenicOvalbuminReceptorsIgGT-Lymphocyte Subsets
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Publication Info
- Year
- 2002
- Type
- article
- Volume
- 168
- Issue
- 8
- Pages
- 3697-3701
- Citations
- 180
- Access
- Closed
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Cite This
Charles Anderson,
David M. Mosser
(2002).
Cutting Edge: Biasing Immune Responses by Directing Antigen to Macrophage Fcγ Receptors.
The Journal of Immunology
, 168
(8)
, 3697-3701.
https://doi.org/10.4049/jimmunol.168.8.3697
Identifiers
- DOI
- 10.4049/jimmunol.168.8.3697
- PMID
- 11937518