Abstract
CRISPOR.org is a web tool for genome editing experiments with the CRISPR-Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.
Keywords
BiologyCRISPRGenome editingSelection (genetic algorithm)Computational biologyGeneticsCas9GenomeGeneComputer scienceArtificial intelligence
MeSH Terms
Base SequenceCRISPR-Associated Protein 9CRISPR-Cas SystemsGene EditingGene Knockout TechniquesGenomeInternetMutagenesisOligonucleotidesRNASoftwareWorkflow
Affiliated Institutions
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Publication Info
- Year
- 2018
- Type
- article
- Volume
- 46
- Issue
- W1
- Pages
- W242-W245
- Citations
- 2177
- Access
- Closed
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Cite This
Jean‐Paul Concordet,
Maximilian Haeussler
(2018).
CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens.
Nucleic Acids Research
, 46
(W1)
, W242-W245.
https://doi.org/10.1093/nar/gky354
Identifiers
- DOI
- 10.1093/nar/gky354
- PMID
- 29762716
- PMCID
- PMC6030908