Abstract
Cellular interactions in adoptive secondary anti-hapten antibody responses to the hapten 2, 4-dinitrophenyl (DNP) have been studied. It was shown that DNP-specific B cells must interact with carrier specific helper T cells to give optimal responses. Independent titration of B cell and helper cell activity in adoptive anti-DNP antibody responses gave the following results: Doubling the number of transferred B cells approximately doubled the subsequent antibody response. Doubling the number of helper cells leads to nearly 4 times as much anti-DNP antibody, measured 7 days after boosting ("premium effect"). This marked effect of helper cell number on the antibody response is thought to be due primarly to the interaction of two populations of carrier-specific cells in the helper effect, or to the interaction of two activities of a single population of helper cells, namely clone activation and clone expansion. Only a very small proportion of the premium effect given by helper cells could be attributed to increases in antibody affinity.
Keywords
Haptenclone (Java method)AntibodyPopulationImmunologyAdoptive cell transferIn vivoB cellDinitrophenylCellBiologyMolecular biologyChemistryCell biologyT cellMedicineBiochemistryImmune systemGenetics
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Publication Info
- Year
- 1975
- Type
- article
- Volume
- 114
- Issue
- 4
- Pages
- 1394-401
- Citations
- 76
- Access
- Closed
External Links
Citation Metrics
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Cite This
Charles A. Janeway
(1975).
Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response..
PubMed
, 114
(4)
, 1394-401.