CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein

Samuel D. Wright; Robert Ramos; Peter S. Tobias; Richard J. Ulevitch; John C. Mathison

doi:10.1126/science.1698311

Abstract

Leukocytes respond to lipopolysaccharide (LPS) at nanogram per milliliter concentrations with secretion of cytokines such as tumor necrosis factor-α (TNF-α). Excess secretion of TNF-α causes endotoxic shock, an often fatal complication of infection. LPS in the bloodstream rapidly binds to the serum protein, lipopolysaccharide binding protein (LBP), and cellular responses to physiological levels of LPS are dependent on LBP. CD14, a differentiation antigen of monocytes, was found to bind complexes of LPS and LBP, and blockade of CD14 with monoclonal antibodies prevented synthesis of TNF-α by whole blood incubated with LPS. Thus, LPS may induce responses by interacting with a soluble binding protein in serum that then binds the cell surface protein CD14.

Keywords

Lipopolysaccharide binding proteinCD14LipopolysaccharideTumor necrosis factor alphaSecretionReceptorAntibodyMonoclonal antibodyChemistryBinding proteinMolecular biologyBiologyImmunologyEndocrinologyBiochemistry

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Publication Info

Year: 1990
Type: article
Volume: 249
Issue: 4975
Pages: 1431-1433
Citations: 3992
Access: Closed

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APA Style

                            
                                
                                    Samuel D. Wright, 
                                
                                    Robert Ramos, 
                                
                                    Peter S. Tobias
                                
                                et al.
                            
                            (1990). 
                            CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein. 
                            Science
                            , 249
                            (4975)
                            , 1431-1433.
                            https://doi.org/10.1126/science.1698311
                        

Identifiers

DOI: 10.1126/science.1698311