Applications of mass spectrometry to DNA sequencing

K. Bruce Jacobson; Heinrich F. Arlinghaus; Michelle V. Buchanan; C.H. Chen; Gary L. Glish; Robert L. Hettich; Scott A. McLuckey

doi:10.1016/1050-3862(91)90032-m

Abstract

The ability of the mass spectrometer to analyze collectively the masses of DNA fragments that are produced in the Sanger procedure for sequencing may allow the gel electrophoresis step to be eliminated. On the other hand, if gel electrophoresis is required, the use of resonance ionization spectroscopy coupled to a mass spectrometer may enable much faster analysis of DNA bands labeled with stable isotopes. Other combinations of labeling of the DNA and its mass spectrometric analysis with or without gel electrophoresis are also considered. Recent advances in these areas of mass spectrometry are reviewed.

Keywords

Mass spectrometrySanger sequencingChemistryGel electrophoresisDNAChromatographyProtein mass spectrometryElectrophoresisDNA sequencingAnalytical Chemistry (journal)Tandem mass spectrometryBiochemistry

MeSH Terms

Base SequenceDNAMass Spectrometry

Affiliated Institutions

Atom Sciences US

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Publication Info

Year: 1991
Type: review
Volume: 8
Issue: 8
Pages: 223-229
Citations: 23
Access: Closed

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APA Style

                            
                                
                                    K. Bruce Jacobson, 
                                
                                    Heinrich F. Arlinghaus, 
                                
                                    Michelle V. Buchanan
                                
                                et al.
                            
                            (1991). 
                            Applications of mass spectrometry to DNA sequencing. 
                            Genetic Analysis Biomolecular Engineering
                            , 8
                            (8)
                            , 223-229.
                            https://doi.org/10.1016/1050-3862(91)90032-m
                        

Identifiers

DOI: 10.1016/1050-3862(91)90032-m
PMID: 1817574

Data Quality

Data completeness: 81%