Abstract
Abstract The molecular masses of electroblotted proteins were determined with a time‐of‐flight mass spectrometer by matrix‐assisted laser desorption/ionization directly from blot membranes. Therefore standard proteins, separated by polyacrylamide gel electrophoresis, were electroblotted onto polyvinylidene difluoride or polyamide membranes by standard procedures. Pieces of membrane containing the protein of interest were soaked in matrix solution and analyzed in the mass spectrometer.
Keywords
Mass spectrometryChemistryChromatographyElectrophoresisMembranePolyamideDesorptionMatrix-assisted laser desorption/ionizationAnalytical Chemistry (journal)Polyacrylamide gel electrophoresisMatrix (chemical analysis)Sample preparation in mass spectrometryElectrospray ionizationPolymer chemistryBiochemistryOrganic chemistryAdsorption
MeSH Terms
AdsorptionAir IonizationAnimalsCattleElectrophoresisPolyacrylamide GelLasersMass SpectrometryProteinsSpectrophotometryInfrared
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Publication Info
- Year
- 1992
- Type
- article
- Volume
- 13
- Issue
- 1
- Pages
- 664-665
- Citations
- 118
- Access
- Closed
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Cite This
Christoph Eckerskorn,
Kerstin Strupat,
Michael Karas
et al.
(1992).
Mass spectrometric analysis of blotted proteins after gel electrophoretic separation by matrix‐assisted laser desorption/ionization.
Electrophoresis
, 13
(1)
, 664-665.
https://doi.org/10.1002/elps.11501301140
Identifiers
- DOI
- 10.1002/elps.11501301140
- PMID
- 1459087