Abstract
Upon cellular DNA damage, the p53 tumor suppressor protein transmits a signal to genes that control the cell cycle and apoptosis. One function of p53 that is important for its role in this pathway is its ability to function as a sequence-specific transcriptional activator. We demonstrate here that short single DNA strands can markedly stimulate the ability of human and murine p53 proteins to bind specifically to a p53 response element in supercoiled DNA. We also show that single-stranded DNA does not stimulate binding by a truncated p53 that lacks the C-terminal domain. Finally, we establish that a peptide spanning the p53 C-terminus has the ability in trans to stimulate sequence-specific DNA binding by p53 dramatically. These data taken together suggest a model in which the p53 C-terminus can recognize DNA structures resulting from damage-induced lesions, and this interaction can be propagated to regulate positively p53 sequence-specific DNA binding.
Keywords
BiologyDNADNA damageCell biologyC-terminusDNA-binding proteinMolecular biologyGeneGeneticsTranscription factorAmino acid
MeSH Terms
AnimalsBase SequenceBinding SitesConsensus SequenceDNA PrimersDNASingle-StrandedDNASuperhelicalDeoxyribonuclease IGenesp53HumansMolecular Sequence DataOligodeoxyribonucleotidesRecombinant ProteinsSequence DeletionSpodopteraTransfectionTumor Suppressor Protein p53
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Publication Info
- Year
- 1995
- Type
- article
- Volume
- 81
- Issue
- 7
- Pages
- 1021-1029
- Citations
- 345
- Access
- Closed
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Cite This
Lata Jayaraman,
Carol Prives
(1995).
Activation of p53 sequence-specific DNA bindingby short single strands of DNA requires the p53 C-terminus.
Cell
, 81
(7)
, 1021-1029.
https://doi.org/10.1016/s0092-8674(05)80007-8
Identifiers
- DOI
- 10.1016/s0092-8674(05)80007-8
- PMID
- 7600571