Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Abstract

The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which, among other things, opens up the possibility of studying the biological relevance of small expression differences.

Keywords

Housekeeping geneNormalization (sociology)BiologyDatabase normalizationComputational biologyGeneGene expression profilingGene expressionMicroarray analysis techniquesGeneticsData miningBioinformaticsComputer scienceArtificial intelligencePattern recognition (psychology)

Affiliated Institutions

Ghent University Hospital BE

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Publication Info

Year: 2002
Type: article
Volume: 3
Issue: 7
Pages: RESEARCH0034-RESEARCH0034
Citations: 19562
Access: Closed

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APA Style

                            
                                
                                    Jo Vandesompele, 
                                
                                    Katleen De Preter, 
                                
                                    Filip Pattyn
                                
                                et al.
                            
                            (2002). 
                            Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. 
                            Genome biology
                            , 3
                            (7)
                            , RESEARCH0034-RESEARCH0034.
                            https://doi.org/10.1186/gb-2002-3-7-research0034
                        

Identifiers

DOI: 10.1186/gb-2002-3-7-research0034