A survey of human brain transcriptome diversity at the single cell level

Abstract

Significance The brain comprises an immense number of cells and cellular connections. We describe the first, to our knowledge, single cell whole transcriptome analysis of human adult cortical samples. We have established an experimental and analytical framework with which the complexity of the human brain can be dissected on the single cell level. Using this approach, we were able to identify all major cell types of the brain and characterize subtypes of neuronal cells. We observed changes in neurons from early developmental to late differentiated stages in the adult. We found a subset of adult neurons which express major histocompatibility complex class I genes and thus are not immune privileged.

Keywords

TranscriptomeHuman brainBiologyMajor histocompatibility complexCellNeuroscienceCell typeComputational biologyGeneGeneticsGene expression

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Publication Info

Year: 2015
Type: article
Volume: 112
Issue: 23
Pages: 7285-7290
Citations: 1485
Access: Closed

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APA Style

                            
                                
                                    Spyros Darmanis, 
                                
                                    Steven A. Sloan, 
                                
                                    Ye Zhang
                                
                                et al.
                            
                            (2015). 
                            A survey of human brain transcriptome diversity at the single cell level. 
                            Proceedings of the National Academy of Sciences
                            , 112
                            (23)
                            , 7285-7290.
                            https://doi.org/10.1073/pnas.1507125112
                        

Identifiers

DOI: 10.1073/pnas.1507125112