A Sequence in M13 Phage Detects Hypervariable Minisatellites in Human and Animal DNA

Abstract

The term "DNA fingerprint" has been used to describe the extensive restriction fragment length polymorphism associated with hypervariable minisatellites present in the human genome. Until now, it was necessary to hybridize Southern blots to specific probes cloned from human genomic DNA in order to obtain individual-specific restriction patterns. The present study describes the surprising finding that the insert-free, wild-type M13 bacteriophage detects hypervariable minisatellites in human and in animal DNA, provided no competitor DNA is used during hybridization. The effective sequence in M13 was traced to two clusters of 15-base pair repeats within the protein III gene of the bacteriophage. This unexpected use of M13 renders the DNA fingerprinting technology more readily available to molecular biology laboratories.

Keywords

MinisatelliteBiologyGeneticsHypervariable regionDNAHuman genomeBacteriophageSouthern blotGenomeGeneMicrosatellite

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Publication Info

Year: 1987
Type: article
Volume: 235
Issue: 4789
Pages: 683-684
Citations: 574
Access: Closed

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APA Style

                            
                                
                                    Gilbert Vassart, 
                                
                                    Michel Georges, 
                                
                                    Rita Monsieur
                                
                                et al.
                            
                            (1987). 
                            A Sequence in M13 Phage Detects Hypervariable Minisatellites in Human and Animal DNA. 
                            Science
                            , 235
                            (4789)
                            , 683-684.
                            https://doi.org/10.1126/science.2880398
                        

Identifiers

DOI: 10.1126/science.2880398