Earl R. Stadtman

[49] Determination of carbonyl content in oxidatively modified proteins

Age-related changes in oxidized proteins.

We have previously described the oxidative inactivation of several key metabolic enzymes by a variety of mixed function oxidation systems. Because many of the enzymes which are ...

Conversion of Amino Acid Residues in Proteins and Amino Acid Homopolymers to Carbonyl Derivatives by Metal-catalyzed Oxidation Reactions

A number of metal-catalyzed oxidation (MCO) systems mediate the oxidative inactivation of enzymes. This oxidation is accompanied by conversion of the side chains of some amino a...

Oxidation of proteins by mixed-function oxidation systems: implication in protein turnover, ageing and neutrophil function

Use of fluorescein hydrazide and fluorescein thiosemicarbazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized protein on polyacrylamide gels

Highly fluorescent thiosemicarbazide and hydrazide prepared by reaction of fluorescein isothiocyanate with hydrazine or adipic acid dihydrazide have been used to monitor the pre...

Modification of hepatic proteins in rats exposed to high oxygen concentration

The effect of high concentrations of oxygen on cellular proteins was examined in hepatocytes isolated from rats exposed to 100% oxygen for 0, 24, 48, or 54 h. Previous studies h...

A Role of Mixed‐Function Oxidation Reactions in the Accumulation of Altered Enzyme Forms During Aging

Protein Oxidation and Aging

A number of systems that generate oxygen free radicals catalyze the oxidative modification of proteins. Such modifications mark enzymes for degradation by cytosolic neutral alka...

Reversible oxidation and inactivation of the tumor suppressor PTEN in cells stimulated with peptide growth factors

Stimulation of cells with various peptide growth factors induces the production of phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ) through activation of phosphatidylinositol 3...