Abstract
Thermodynamics of 23 oligonucleotides with internal single C.T mismatches were obtained by measuring UV absorbance as a function of temperature. Results from these 23 duplexes were combined with three measurements from the literature to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal C.T mismatches. The data show that the nearest-neighbor model is adequate for predicting thermodynamics of oligonucleotides with internal C.T with average deviations for Delta G degrees37, Delta H degrees, Delta S degrees and T m of 6.4%, 9.9%, 10.6%, and 1.9 degreesC respectively. C.T mismatches destabilize the duplex in all sequence contexts. The thermodynamic contribution of C. T mismatches to duplex stability varies weakly depending on the orientation of the mismatch and its context and ranges from +1.02 kcal/mol for GCG/CTC and CCG/GTC to +1.95 kcal/mol for TCC/ATG.
Keywords
Duplex (building)ThermodynamicsOligonucleotideContext (archaeology)TrimerPhysicsDNABiologyNuclear magnetic resonanceBiochemistryDimer
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Publication Info
- Year
- 1998
- Type
- article
- Volume
- 26
- Issue
- 11
- Pages
- 2694-2701
- Citations
- 236
- Access
- Closed
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Cite This
Hatim T. Allawi
(1998).
Thermodynamics of internal C.T mismatches in DNA.
Nucleic Acids Research
, 26
(11)
, 2694-2701.
https://doi.org/10.1093/nar/26.11.2694
Identifiers
- DOI
- 10.1093/nar/26.11.2694