Abstract
A naturally occurring small RNA molecule ( micF RNA), complementary to the region encompassing the Shine-Dalgarno sequence and initiation codon of the ompF mRNA, is known to block the expression of that mRNA in E. coli. We have constructed a plasmid that produces a complementary RNA to the E. coli lpp mRNA (mic[Ipp] RNA). Induction of the mic(Ipp) gene efficiently blocked lipoprotein production and reduced the amount of lpp mRNA. Two mic(ompC) genes were similarly engineered and their expression was found to inhibit drastically production of OmpC. Analysis of several types of mic(ompA) genes suggests that micRNAs complementary to regions of the mRNA likely to come in contact with ribosomes were most effective. The novel capabilities of this artificial mic system provide great potential for application in both procaryotic and eucaryotic cells.
Keywords
BiologyGeneRNARibosomeMessenger RNAGene expressionPlasmidRibosomal binding siteStart codonGeneticsMolecular biologyCell biology
MeSH Terms
Bacterial ProteinsBase SequenceCodonDNA Restriction EnzymesEscherichia coliGenesBacterialGenetic VectorsPlasmidsRNARNABacterialRNAMessengerRNASmall NuclearTranscriptionGenetic
Affiliated Institutions
Related Publications
The 3′-terminal sequence of the small subunit ribosomal RNA from hamster mitochondria
The 220 3'-terminal nucleotides of the small ribosomal subunit RNA (13S) of hamster (BHK-21) cell mitochondria have been sequenced and the positions of post-transcriptionally me...
Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.
We have determined the complete nucleotide sequence of the gene for the crown gall enzyme, octopine synthase. The sequence was derived from cloned fragments of the Agrobacterium...
Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface
Polypeptide library screening technologies are critically dependent upon the characteristics of the expression system employed. A comparative analysis of the lpp-lac, tet and ar...
Construction of versatile expression cloning vehicles using the lipoprotein gene of Escherichia coli.
The gene for the outer membrane lipoprotein (lpp), the most abundant protein of Escherichia coli, was used to construct multi-purpose expression cloning vehicles. These vehicles...
Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNA
Plasmid DNA directing transcription of the noncoding (anti-sense) DNA strand can specifically inhibit the expression of several test genes as well as normal, endogenous genes. T...
Publication Info
- Year
- 1984
- Type
- article
- Volume
- 37
- Issue
- 2
- Pages
- 429-436
- Citations
- 209
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
209
OpenAlex
3
Influential
153
CrossRef
Cite This
Jack Coleman,
Pamela J. Green,
Masayori Inouye
(1984).
The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes.
Cell
, 37
(2)
, 429-436.
https://doi.org/10.1016/0092-8674(84)90373-8
Identifiers
- DOI
- 10.1016/0092-8674(84)90373-8
- PMID
- 6202422