Abstract

Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature.

Keywords

BiologyEmbryonic stem cellInduced pluripotent stem cellStem cellCell biologyInduced stem cellsGeneticsGene

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Year
2011
Type
article
Volume
7
Issue
1
Pages
550-550
Citations
140
Access
Closed

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Javier Muñoz, Teck Yew Low, Yee Jiun Kok et al. (2011). The quantitative proteomes of human‐induced pluripotent stem cells and embryonic stem cells. Molecular Systems Biology , 7 (1) , 550-550. https://doi.org/10.1038/msb.2011.84

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DOI
10.1038/msb.2011.84