Keywords
Affiliated Institutions
Related Publications
Ligation-independent cloning of PCR products (LIC-PCR)
A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are gener...
Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments
Gapped duplex DNA molecules of recombinant genomes of filamentous phage are constructed in vitro. Denatured restriction fragments covering (part of) the precisely constructed ga...
In vivo packaging of cosmids in transposon-mediated mutagenesis
A technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host ...
Peptide nucleic acid-targeted mutagenesis of a chromosomal gene in mouse cells
Peptide nucleic acids (PNAs) can bind to single-stranded DNA by Watson–Crick base pairing and can form triple helices via Hoogsteen bonding to DNA/PNA duplexes. A single dimeric...
Insertional Mutagenesis of the <i>Drosophila</i> Genome with Single P Elements
A versatile genetic method for identifying and cloning Drosophila melanogaster genes affecting any recognizable phenotype is described. Strains are constructed in which the inse...
Publication Info
- Year
- 1982
- Type
- article
- Volume
- 19
- Issue
- 3
- Pages
- 259-268
- Citations
- 6129
- Access
- Closed
External Links
Social Impact
Social media, news, blog, policy document mentions
Citation Metrics
Cite This
Identifiers
- DOI
- 10.1016/0378-1119(82)90015-4