Abstract
Caenorhabditis elegans let-7 , a founding member of the microRNA family, is predicted to bind to six sites in the 3′UTR of the mRNA of its target gene, lin-41 , to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3′UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3′UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.
Keywords
BiologyUntranslated regionCaenorhabditis elegansThree prime untranslated regionmicroRNAGeneticsMessenger RNAGeneFive prime untranslated region
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Publication Info
- Year
- 2004
- Type
- article
- Volume
- 18
- Issue
- 2
- Pages
- 132-137
- Citations
- 444
- Access
- Closed
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Cite This
Monica C. Vella,
Eun Young Choi,
Shin-Yi Lin
et al.
(2004).
The <i>C. elegans</i> microRNA <i>let-7</i> binds to imperfect <i>let-7</i> complementary sites from the <i>lin-41</i> 3′UTR.
Genes & Development
, 18
(2)
, 132-137.
https://doi.org/10.1101/gad.1165404
Identifiers
- DOI
- 10.1101/gad.1165404