The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

Abstract

Abstract The focusing positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins calculated from their amino acid sequences matched with good accuracy the experimentally determined p I values. We show the advantages of being able to predict the position of a protein of known structure within a two‐dimensional gel.

Keywords

Isoelectric focusingImmobilized pH gradientIsoelectric pointAmino acidChromatographyChemistryAmino acid residuePeptide sequenceBiochemistryEnzyme

Affiliated Institutions

University of Geneva CH

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Publication Info

Year: 1993
Type: article
Volume: 14
Issue: 1
Pages: 1023-1031
Citations: 1002
Access: Closed

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APA Style

                            
                                
                                    Bengt Bjellqvist, 
                                
                                    Graham J. Hughes, 
                                
                                    Christian Pasquali
                                
                                et al.
                            
                            (1993). 
                            The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. 
                            Electrophoresis
                            , 14
                            (1)
                            , 1023-1031.
                            https://doi.org/10.1002/elps.11501401163
                        

Identifiers

DOI: 10.1002/elps.11501401163