Abstract
The eukaryotic protein kinases make up a large superfamily of homologous proteins. They are related by virtue of their kinase domains (also known as catalytic domains), which consist of ≈ 250‐300 amino acid residues. The kinase domains that define this group of enzymes contain 12 conserved subdomains that fold into a common catalytic core structure, as revealed by the 3‐dimensional structures of severed protein‐serine kinases. There are two main subdivisions within the superfamily: the protein‐serine/threonine kinases and the protein‐tyrosine kinases. A classification scheme can be founded on a kinase domain phylogeny, which reveals families of enzymes that have related substrate specificities and modes of regulation.—Hanks, S. K., Hunter, T. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. FASEB J. 9, 576‐596 (1995)
Keywords
Protein superfamilyBiochemistryc-RafKinaseProtein kinase domainMAP2K7SH3 domainBiologySerineProtein kinase AChemistryCyclin-dependent kinase 2EnzymeReceptor tyrosine kinaseGene
MeSH Terms
Amino Acid SequenceBinding SitesCatalysisEukaryotic CellsForecastingHumansMolecular Sequence DataProtein ConformationProtein KinasesSequence HomologyAmino Acid
Affiliated Institutions
Related Publications
Crystal Structure of the Atypical Protein Kinase Domain of a TRP Channel with Phosphotransferase Activity
Transient receptor potential (TRP) channels modulate calcium levels in eukaryotic cells in response to external signals. A novel transient receptor potential channel has the abi...
Oncogenic kinase signalling
Protein-tyrosine kinases (PTKs) are important regulators of intracellular signal-transduction pathways mediating development and multicellular communication in metazoans. Their ...
Gβγ Subunits Mediate Src-dependent Phosphorylation of the Epidermal Growth Factor Receptor
In many cells, stimulation of mitogen-activated protein kinases by both receptor tyrosine kinases and receptors that couple to pertussis toxin-sensitive heterotrimeric G protein...
WNK1, a Novel Mammalian Serine/Threonine Protein Kinase Lacking the Catalytic Lysine in Subdomain II
We have cloned and characterized a novel mammalian serine/threonine protein kinase WNK1 (with no lysine (K)) from a rat brain cDNA library. WNK1 has 2126 amino acids and can be ...
Insect cell-expressed p180erbB3 possesses an impaired tyrosine kinase activity.
Protein kinases share a number of highly conserved or invariant amino acid residues in their catalytic domains, suggesting that these residues are necessary for kinase activity....
Publication Info
- Year
- 1995
- Type
- article
- Volume
- 9
- Issue
- 8
- Pages
- 576-596
- Citations
- 2278
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
2278
OpenAlex
83
Influential
2234
CrossRef
Cite This
Steven K. Hanks,
Tony Hunter
(1995).
The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification <sup>1</sup>.
The FASEB Journal
, 9
(8)
, 576-596.
https://doi.org/10.1096/fasebj.9.8.7768349
Identifiers
- DOI
- 10.1096/fasebj.9.8.7768349
- PMID
- 7768349