Abstract
CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agriculture. CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin engineering, and imaging, now exceed the gene-editing functionality of WT Cas9. Here, we will present a brief history of gene-editing tools and describe the wide range of CRISPR-based genome-targeting tools. We will conclude with future directions and the broader impact of CRISPR technologies.CRISPR has rapidly become an indispensable tool for biological research. Here Mazhar Adli reviews the current toolbox for editing and manipulating the genome and looks toward future developments in this fast moving field.
Keywords
CRISPRGenome editingCas9Computational biologyGenomeEpigeneticsGeneBiologyChromatinComputer scienceGenetics
MeSH Terms
AnimalsCRISPR-Cas SystemsClustered Regularly Interspaced Short Palindromic RepeatsGene EditingHistory20th CenturyHistory21st CenturyHumans
Affiliated Institutions
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Publication Info
- Year
- 2018
- Type
- review
- Volume
- 9
- Issue
- 1
- Pages
- 1911-1911
- Citations
- 1561
- Access
- Closed
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Cite This
Mazhar Adli
(2018).
The CRISPR tool kit for genome editing and beyond.
Nature Communications
, 9
(1)
, 1911-1911.
https://doi.org/10.1038/s41467-018-04252-2
Identifiers
- DOI
- 10.1038/s41467-018-04252-2
- PMID
- 29765029
- PMCID
- PMC5953931