Target mRNA abundance dilutes microRNA and siRNA activity

Aaron Arvey; Erik Larsson; Chris Sander; Christina S. Leslie; Debora S. Marks

doi:10.1038/msb.2010.24

Keywords

microRNABiologySmall interfering RNARNA interferenceComputational biologyMessenger RNAGene expressionGeneTrans-acting siRNARegulation of gene expressionSmall RNARNACell biologyGene silencingDownregulation and upregulationTransfectionGenetics

Affiliated Institutions

Related Publications

A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

Thijn R. Brummelkamp , René Bernards , Reuven Agami

Mammalian genetic approaches to study gene function have been hampered by the lack of tools to generate stable loss-of-function phenotypes efficiently. We report here a new vect...

2002 Science 4288 citations

Determinants of targeting by endogenous and exogenous microRNAs and siRNAs

Cydney Nielsen , Noam Shomron , Rickard Sandberg +3 more

Vertebrate mRNAs are frequently targeted for post-transcriptional repression by microRNAs (miRNAs) through mechanisms involving pairing of 3′ UTR seed matches to bases at the 5′...

2007 RNA 384 citations

Therapeutic siRNA: state of the art

Bo Hu , Liping Zhong , Yuhua Weng +4 more

ABSTRACT RNA interference (RNAi) is an ancient biological mechanism used to defend against external invasion. It theoretically can silence any disease-related genes in a sequenc...

2020 Signal Transduction and Targeted Therapy 1344 citations

Experimental validation of the importance of seed complement frequency to siRNA specificity

Emily M. Anderson , Amanda Birmingham , Scott Baskerville +6 more

Pairing between the hexamer seed region of a small interfering RNA (siRNA) guide strand (nucleotides 2–7) and complementary sequences in the 3′ UTR of mature transcripts has bee...

2008 RNA 144 citations

Systematic Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance

David G. Hendrickson , Daniel J. Hogan , Daniel Herschlag +2 more

microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understand...

2008 PLoS ONE 171 citations

Publication Info

Year: 2010
Type: article
Volume: 6
Issue: 1
Pages: 363-363
Citations: 335
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Target mRNA abundance dilutes microRNA and siRNA activity

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

335

OpenAlex

Cite This

APA Style

                            
                                
                                    Aaron Arvey, 
                                
                                    Erik Larsson, 
                                
                                    Chris Sander
                                
                                et al.
                            
                            (2010). 
                            Target mRNA abundance dilutes microRNA and siRNA activity. 
                            Molecular Systems Biology
                            , 6
                            (1)
                            , 363-363.
                            https://doi.org/10.1038/msb.2010.24
                        

Identifiers

DOI: 10.1038/msb.2010.24