Abstract
We used an antiserum against purified cholinergic synaptic vesicles from Torpedo and expression screening to isolate a cDNA clone encoding synuclein, a 143 amino acid neuron-specific protein. A cDNA clone was also isolated from a rat brain cDNA library that encodes a highly homologous 140 amino acid protein. The amino terminal 100 amino acids of both proteins are comprised of an 11 amino acid repeating unit that contains a conserved core of 6 residues. The synuclein gene is expressed only in nervous system tissue, not in electric organ, muscle, liver, spleen, heart, or kidney. In the electric organ synapse Torpedo synuclein-immunoreactive proteins are found in 3 major molecular-weight classes of 17.5, 18.5, and 20.0 kDa. In the neuronal cell soma the 17.5 kDa species is predominant and immunoreactivity is localized to a portion of the nuclear envelope.
Keywords
Complementary DNAcDNA libraryBiologyAmino acidSynaptic vesicleNeuronMolecular biologyCell biologyPeptide sequenceTorpedoBiochemistryGeneNeuroscienceVesicleAcetylcholine receptor
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Publication Info
- Year
- 1988
- Type
- article
- Volume
- 8
- Issue
- 8
- Pages
- 2804-2815
- Citations
- 1584
- Access
- Closed
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Cite This
Luc Maroteaux,
JT Campanelli,
RH Scheller
(1988).
Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal.
Journal of Neuroscience
, 8
(8)
, 2804-2815.
https://doi.org/10.1523/jneurosci.08-08-02804.1988
Identifiers
- DOI
- 10.1523/jneurosci.08-08-02804.1988