Abstract
Abstract In order to study the signal transduction mechanism of human endothelial cells (EC), the regulation of superoxide anion (O 2 − ) release in EC has been investigated using the calcium ionophore A23187 and phorbol myristate acetate (PMA), a potential activator of the Ca 2+ activated, phospholipid‐dependent protein kinase, designated “protein kinase C.” PMA enhanced O 2 − release from EC, and this enhancement occurred regardless of the presence or absence of extracellular Ca 2+ . A similar increase was produced by A23187; omission of extracellular Ca 2+ prevented this increase. Simultaneous stimulation with PMA and A23187 produced a large increase in O 2 − release at submaximal concentrations of these agents, which, when added separately, caused minimal effects. These findings indicate that the activation of protein kinase C and mobilization of Ca 2+ evoked by PMA and A23187 respectively are synergistically effective for eliciting a full physiological response of EC in the generation and release of O 2 − .
Keywords
IonophoreProtein kinase CExtracellularCalciumSuperoxideChemistryActivator (genetics)PhorbolStimulationBiochemistryProtein kinase AKinaseBiophysicsEndocrinologyBiologyEnzymeReceptor
Affiliated Institutions
Related Publications
Calcium, phospholipid turnover and transmembrane signalling
Turnover of phosphatidylinositol, which is provoked by various neurotransmitters, peptide hormones and many other biologically active substances, appears to serve as a signal fo...
Big Mitogen-activated Protein Kinase 1 (BMK1) Is a Redox-sensitive Kinase
Mitogen-activated protein (MAP) kinases are a multigene family activated by many extracellular stimuli. There are three groups of MAP kinases based on their dual phosphorylation...
Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells.
Vascular endothelial growth factor (VEGF) is a regulator of vasculogenesis and angiogenesis. To investigate the role of nitric oxide (NO) in VEGF-induced proliferation and in vi...
Expression and Modulation of an NADPH Oxidase in Mammalian Astrocytes
Amyloid β peptides generate oxidative stress in hippocampal astrocytes through a mechanism sensitive to inhibitors of the NADPH oxidase [diphenylene iodonium (DPI) and apocynin]...
Effect of age on kinetics of nitric oxide release in rat aorta and pulmonary artery.
Aging is an important determinant of vascular disease. Endothelium-derived nitric oxide (NO) is protective as a vasodilator and inhibitor of platelet function. This study was de...
Publication Info
- Year
- 1986
- Type
- article
- Volume
- 127
- Issue
- 2
- Pages
- 207-210
- Citations
- 165
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
165
OpenAlex
Cite This
Tsukasa Matsubara,
Morris Ziff
(1986).
Superoxide anion release by human endothelial cells: Synergism between a phorbol ester and a calcium ionophore.
Journal of Cellular Physiology
, 127
(2)
, 207-210.
https://doi.org/10.1002/jcp.1041270203
Identifiers
- DOI
- 10.1002/jcp.1041270203