Abstract

Abstract HeLa cells were used in the mixed agglutination reaction to determine optimal conditions for demonstrating blood group H activity by this method. The following parameters were studied in the mixed agglutination reaction: (1) derivation of cell line, (2) cell viability, (3) effects of antibody titer, (4) source and type of antibody. Studies with primary human amnion cells indicated that over a 30-day period of cultivation in vitro there were losses in specific ABO blood group activity. Addition of blood group precursors to establish human amnion cell lines FL-J and F-D indicated that blood group B antigen could be synthesized and maintained in vitro.

Keywords

ABO blood group systemAgglutination (biology)AmnionAntibodyHeLaIn vitroTiterImmunologyAndrologyCell cultureGroup AAntigenBiologyHuman bloodMolecular biologyBlood cellMedicineInternal medicineBiochemistryPhysiology

MeSH Terms

ABO Blood-Group SystemAmnionBlood Group AntigensCulture MediaGeneticsPopulationHeLa CellsHemagglutinationHemagglutination TestsHumansPlant LectinsResearchTissue Culture Techniques

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Publication Info

Year
1965
Type
article
Volume
25
Issue
6
Pages
944-953
Citations
38
Access
Closed

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Cite This

Lawrence N. Chessin, Sharon D. Bramson, William J. Kuhns et al. (1965). Studies on the A, B, O(H) Blood Groups on Human Cells in Culture. Blood , 25 (6) , 944-953. https://doi.org/10.1182/blood.v25.6.944.944

Identifiers

DOI
10.1182/blood.v25.6.944.944
PMID
14294771

Data Quality

Data completeness: 86%