Standard reporting requirements for biological samples in metabolomics experiments: microbial and in vitro biology experiments

Abstract

With the increasing use of metabolomics as a means to study a large number of different biological research questions, there is a need for a minimal set of reporting standards that allow the scientific community to evaluate, understand, repeat, compare and re-investigate metabolomics studies. Here we propose, a first draft of minimal requirements to effectively describe the biological context of metabolomics studies that involve microbial or in vitro biological subjects. This recommendation has been produced by the microbiology and in vitro biology working subgroup of the Metabolomics Standards Initiative in collaboration with the yeast systems biology network as part of a wider standardization initiative led by the Metabolomics Society. Microbial and in vitro biology metabolomics is defined by this sub-working group as studies with any cell or organism that require a defined external medium to facilitate growth and propagation. Both a minimal set and a best practice set of reporting standards for metabolomics experiments have been defined. The minimal set of reporting standards for microbial or in vitro biology metabolomics experiments includes those factors that are <i>specific</i> for metabolomics experiments and that critically determine the outcome of the experiments. The best practice set of reporting standards contains both the factors that are <i>specific</i> for metabolomics experiments and <i>general aspects</i> that critically determine the outcome of any microbial or in vitro biological experiment.

Keywords

Affiliated Institutions

• Klinikum Hanau DE
• Technical University of Denmark DK
• National Institute for Environmental Studies JP
• The University of Sydney AU
• Forschungszentrum Jülich DE
• Institut National des Sciences Appliquées de Toulouse FR
• Sigma Tau (Italy) IT
• Saarland University DE
• Bangor University GB
• ETH Zurich CH
• Quality of Life Research Center DK

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