Spatial control of <i>hairy</i> protein expression during embryogenesis

Abstract

ABSTRACT We have used a polyclonal antiserum specific for the Drosophila segmentation gene, hairy (h), to analyse its expression during embryogenesis. The pattern of wildtype expression resembles that of h transcription, being expressed in stripes at the blastoderm stage, h is also expressed later in the stomodæum, proctodæum, tracheal pits and mesoderm. We demonstrate that h protein stripes show consistent phase relationships to those of the even-skipped (eve) pair-rule gene. We examine h protein patterns in embryos mutant for other segmentation genes, including h itself. We show that lack of h activity appears not to affect h striping, arguing that h expression is not under autoregulatory control. We also show that h activity is not needed for tracheal invagination. Mutations that are rearranged upstream of the h gene cause the loss of specific stripes, indicating that the h promoter includes activating elements that respond to specific spatial cues. Our observations suggest that pairrule striping may be under redundant control, and we discuss possible implications for hierarchical models of pair-rule gene action.

Keywords

Affiliated Institutions

• University of Oxford GB

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