Abstract
Oligonucleotides equipped with EDTA⋅Fe can bind specifically to duplex DNA by triple-helix formation and produce double-strand cleavage at binding sites greater than 12 base pairs in size. To demonstrate that oligonucleotide-directed triple-helix formation is a viable chemical approach for the site-specific cleavage of large genomic DNA, an oligonucleotide with EDTA⋅Fe at the 5′ and 3′ ends was targeted to a 20-base pair sequence in the 340-kilobase pair chromosome III of Saccharomyces cerevisiae . Double-strand cleavage products of the correct size and location were observed, indicating that the oligonucleotide bound and cleaved the target site among almost 14 megabase pairs of DNA. Because oligonucleotide-directed triple-helix formation has the potential to be a general solution for DNA recognition, this result has implications for physical mapping of chromosomes.
Keywords
Triple helixOligonucleotideCleavage (geology)DNADuplex (building)BiologyBase pairMolecular biologyGenetics
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Publication Info
- Year
- 1990
- Type
- article
- Volume
- 249
- Issue
- 4964
- Pages
- 73-75
- Citations
- 197
- Access
- Closed
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Cite This
Scott A. Strobel,
Peter B. Dervan
(1990).
Site-Specific Cleavage of a Yeast Chromosome by Oligonucleotide-Directed Triple-Helix Formation.
Science
, 249
(4964)
, 73-75.
https://doi.org/10.1126/science.2195655
Identifiers
- DOI
- 10.1126/science.2195655